1ZI0

A Superhelical Spiral in Escherichia coli DNA Gyrase A C-terminal Domain Imparts Unidirectional Supercoiling Bias

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-01-20
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9360
Spacegroup name	I 2 2 2
Unit cell lengths	104.216, 121.432, 177.957
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.300 - 2.600
Rwork	0.227
R-free	0.26600
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.720
High resolution limit [Å]	2.600	2.600
Number of reflections	32428
Completeness [%]	97.8	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Tris, PEG 1500, NaCl, Sodium Phosphate, EDTA, Beta-Mercaptoethanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Tris, PEG 1500, NaCl, Sodium Phosphate, EDTA, Beta-Mercaptoethanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K