1ZHO
The structure of a ribosomal protein L1 in complex with mRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.600, 101.900, 119.600 |
| Unit cell angles | 90.00, 93.66, 90.00 |
Refinement procedure
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 37.060 |
| High resolution limit [Å] | 2.600 |
| Number of reflections | 50877 |
| Completeness [%] | 96.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | KCl, MgCl2, HgCl2, PEG 10000, cacodylate, glycerol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | KCl | ||
| 10 | 1 | 2 | HgCl2 | ||
| 11 | 1 | 2 | PEG 10000 | ||
| 12 | 1 | 2 | cacodylate | ||
| 13 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | HgCl2 | ||
| 4 | 1 | 1 | PEG 10000 | ||
| 5 | 1 | 1 | cacodylate | ||
| 6 | 1 | 1 | glycerol | ||
| 7 | 1 | 1 | H2O | ||
| 8 | 1 | 2 | KCl | ||
| 9 | 1 | 2 | MgCl2 |
