1ZHB
Crystal Structure Of The Murine Class I Major Histocompatibility Complex Of H-2Db, B2-Microglobulin, and a 9-Residue Peptide Derived from rat dopamine beta-monooxigenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-09-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0292 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.063, 122.715, 99.403 |
| Unit cell angles | 90.00, 103.00, 90.00 |
Refinement procedure
| Resolution | 24.920 - 2.700 |
| R-factor | 0.226 |
| Rwork | 0.225 |
| R-free | 0.26176 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n5a |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.393 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 58548 | |
| <I/σ(I)> | 14.4 | 2 |
| Completeness [%] | 98.8 | 93.6 |
| Redundancy | 3.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | ammonium sulphate, Tris HCl, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
