1ZH5

Structural basis for recognition of UUUOH 3'-terminii of nascent RNA pol III transcripts by La autoantigen

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-16
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1000
Spacegroup name	P 21 21 2
Unit cell lengths	156.209, 55.030, 57.782
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.920 - 1.850
R-factor	0.20252
Rwork	0.200
R-free	0.24528
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.681
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.920
High resolution limit [Å]	1.850	1.850
Rmerge		0.496
Number of reflections	42919
<I/σ(I)>	14.9	3.7
Completeness [%]	99.4	99.4
Redundancy	5.3	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	277	PEG 8000, lithium sulfate, potassium chloride, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	4.6	277	PEG 8000, lithium sulfate, potassium chloride, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	4.6	277	PEG 8000, lithium sulfate, potassium chloride, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 8000
10	1	2	H20
2	1	1	lithium sulfate
3	1	1	potassium chloride
4	1	1	sodium acetate
5	1	1	H20
6	1	2	PEG 8000
7	1	2	lithium sulfate
8	1	2	potassium chloride
9	1	2	sodium acetate