1ZFP

GROWTH FACTOR RECEPTOR BINDING PROTEIN SH2 DOMAIN COMPLEXED WITH A PHOSPHOTYROSYL PENTAPEPTIDE

Experimental procedure

Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR571
Temperature [K]	293
Detector technology	DIFFRACTOMETER
Collection date	1994-10-21
Detector	ENRAF-NONIUS FAST
Spacegroup name	C 2 2 21
Unit cell lengths	49.900, 113.700, 46.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1LCJ (LCK-SH2)
RMSD bond length	0.011
RMSD bond angle	22.481 *
Data reduction software	MADNES
Data scaling software	CCP4
Phasing software	TFFC
Refinement software	TNT (4-A)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.850
High resolution limit [Å]	1.800	1.800
Rmerge	0.093	0.440
Number of reflections	12572
<I/σ(I)>	4.6	1.7
Completeness [%]	99.0	98
Redundancy	5.6	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-20 (mg/ml)
2	1	reservoir	ammonium sulfate	2.0 (M)
3	1	reservoir	PEG400	2 (%)
4	1	reservoir	HEPES	0.1 (M)