1ZFN

Structural Analysis of Escherichia coli ThiF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-31
Detector	MARRESEARCH
Wavelength(s)	0.9793
Spacegroup name	F 41 3 2
Unit cell lengths	360.300, 360.300, 360.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.750
Rwork	0.247
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.430
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.870
High resolution limit [Å]	2.750	2.750
Number of reflections	52090
Completeness [%]	99.8	98.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	Ammonium Acetate, MES, DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K