1ZFJ
INOSINE MONOPHOSPHATE DEHYDROGENASE (IMPDH; EC 1.1.1.205) FROM STREPTOCOCCUS PYOGENES
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Detector | SBC-1 |
| Wavelength(s) | 0.9791,1.0781 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 151.480, 151.480, 101.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.900 |
| R-factor | 0.234 * |
| Rwork | 0.232 |
| R-free | 0.26300 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 21.800 * |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.068 | 0.319 |
| Total number of observations | 263355 * | |
| Number of reflections | 44921 | |
| <I/σ(I)> | 6 | 2.5 |
| Completeness [%] | 96.5 | 87.6 |
| Redundancy | 6.2 | 3.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 * | 10 * | 0.1 M MES (PH 7.2), 1.8 M AMMONIUM SULFATE, 10 MM COCL2, pH 7.20 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MES | 0.1 (M) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
| 3 | 1 | reservoir | 10 (mM) | ||
| 4 | 1 | drop | protein | 20 (mg/ml) | |
| 5 | 1 | drop | Bis-Tris-propane | 10 (mM) | |
| 6 | 1 | drop | IMP | 1 (mM) |
