1ZF2

Four-stranded DNA Holliday Junction (CCC)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	103
Detector technology	CCD
Collection date	2004-02-26
Detector	MARRESEARCH
Wavelength(s)	0.827
Spacegroup name	C 1 2 1
Unit cell lengths	64.960, 24.710, 36.760
Unit cell angles	90.00, 110.69, 90.00

Refinement procedure

Resolution	16.000 - 1.950
R-factor	0.231
Rwork	0.231
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NDB ENTRY UD0028
RMSD bond length	0.009
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.000	2.020
High resolution limit [Å]	1.880	1.880
Rmerge	0.103	0.335
Number of reflections	3883
<I/σ(I)>	8	2.2
Completeness [%]	88.9	65.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	Na Cacodylate, CaCl2, Spermine, MPD in resevoir, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	7	298	Na Cacodylate, CaCl2, Spermine, MPD in resevoir, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	Na Cacodylate
10	2	1	Na Cacodylate
12	2	1	CaCl2
13	2	1	Spermine
14	2	1	MPD
15	2	1	H2O
16	2	2	Na Cacodylate
17	2	2	CaCl2
18	2	2	MPD
19	2	2	H2O
2	1	1	CaCl2
3	1	1	Spermine
4	1	1	MPD
5	1	1	H2O
6	1	2	Na Cacodylate
7	1	2	CaCl2
8	1	2	MPD
9	1	2	H2O