1ZDJ

STRUCTURE OF BACTERIOPHAGE COAT PROTEIN-LOOP RNA COMPLEX

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	280
Detector technology	IMAGE PLATE
Collection date	1994-12-12
Detector	MARRESEARCH
Spacegroup name	H 3 2
Unit cell lengths	288.000, 288.000, 653.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.900
R-factor	0.182
Rwork	0.182
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	28.000 *
Data reduction software	DENZO
Data scaling software	CCP4 ((AGROVATA)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.970
High resolution limit [Å]	2.900	2.900
Rmerge	0.114	0.280
Number of reflections	168328
Completeness [%]	74.1	41.2
Redundancy	2.3	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4	37 *	Valegard, K., (1986) J. Mol. Biol., 190, 587. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MS2	1.0 (%(w/v))
2	1	drop	sodium phosphate	0.2 (M)
3	1	drop	PEG6000	1.5 (%(w/v))
4	1	drop		0.02 (%(w/v))
5	1	reservoir	sodium phospahte	0.4 (M)