1ZDI
RNA BACTERIOPHAGE MS2 COAT PROTEIN/RNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 288.000, 288.000, 653.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.700 |
| R-factor | 0.202 * |
| Rwork | 0.202 |
| RMSD bond length | 0.016 * |
| RMSD bond angle | 2.070 * |
| Data reduction software | DENZO |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.100 * | |
| High resolution limit [Å] | 2.700 * | 2.700 * |
| Rmerge | 0.092 | 0.186 * |
| Number of reflections | 193997 | |
| Completeness [%] | 75.3 * | 51.4 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | PEG 8000 | ||
| 3 | 1 | 1 | NA PHOSPHATE | ||
| 4 | 1 | 1 | NA AZIDE | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | NA PHOSPHATE | ||
| 7 | 1 | 2 | NA AZIDE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | capsid | 0.6 (mg/ml) | |
| 2 | 1 | drop | PEG8000 | 1.25 (%(w/v)) | |
| 3 | 1 | drop | sodium phosphate | 0.1 (M) | |
| 4 | 1 | drop | sodium azide | 0.02 (%(w/v)) | |
| 5 | 1 | reservoir | sodium phosphate | 0.4 (M) | |
| 6 | 1 | reservoir | sodium azide | 0.02 (%(w/v)) |
