1ZDI

RNA BACTERIOPHAGE MS2 COAT PROTEIN/RNA COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	H 3 2
Unit cell lengths	288.000, 288.000, 653.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.700
R-factor	0.202 *
Rwork	0.202
RMSD bond length	0.016 *
RMSD bond angle	2.070 *
Data reduction software	DENZO
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.100 *
High resolution limit [Å]	2.700 *	2.700 *
Rmerge	0.092	0.186 *
Number of reflections	193997
Completeness [%]	75.3 *	51.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4 *		VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	WATER
2	1	1	PEG 8000
3	1	1	NA PHOSPHATE
4	1	1	NA AZIDE
5	1	2	WATER
6	1	2	NA PHOSPHATE
7	1	2	NA AZIDE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	capsid	0.6 (mg/ml)
2	1	drop	PEG8000	1.25 (%(w/v))
3	1	drop	sodium phosphate	0.1 (M)
4	1	drop	sodium azide	0.02 (%(w/v))
5	1	reservoir	sodium phosphate	0.4 (M)
6	1	reservoir	sodium azide	0.02 (%(w/v))