1ZCK
native structure prl-1 (ptp4a1)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-06-30 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 0.9800, 0.97849, 0.96261 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 71.291, 105.568, 181.355 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| Rwork | 0.245 |
| R-free | 0.25700 |
| Structure solution method | MAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 48966 | |
| Completeness [%] | 96.9 | 88.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 277 | Ammonium Sulfate, Sodium Acetate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
