1ZC6
Crystal Structure of Putative N-acetylglucosamine Kinase from Chromobacterium violaceum. Northeast Structural Genomics Target Cvr23.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9876 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 178.245, 178.245, 117.663 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.420 - 2.200 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.26700 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.104 | 0.863 |
| Number of reflections | 90884 | |
| Completeness [%] | 95.4 | |
| Redundancy | 10.1 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.15-0.25 M magnesium chloride, 15-20% PEG 3350, 0.1 M MES , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
