1ZBM

X-Ray Crystal Structure of Protein AF1704 from Archaeoglobus fulgidus. Northeast Structural Genomics Consortium Target GR62A.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-07
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97912
Spacegroup name	P 21 21 21
Unit cell lengths	40.784, 69.931, 105.581
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.310 - 2.300
R-factor	0.212
Rwork	0.212
R-free	0.26500
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.089	0.252
Number of reflections	24762
<I/σ(I)>	20.07	8.01
Completeness [%]		99.8
Redundancy	6.1	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	100mM sodium acetate, 20% PEG4k, 200mM ammonium acetate, 5mM DTT., pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K