1ZBD

STRUCTURAL BASIS OF RAB EFFECTOR SPECIFICITY: CRYSTAL STRUCTURE OF THE SMALL G PROTEIN RAB3A COMPLEXED WITH THE EFFECTOR DOMAIN OF RABPHILIN-3A

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-05-31
Detector	RIGAKU
Wavelength(s)	0.9789, 0.9793, 0.9879
Spacegroup name	C 1 2 1
Unit cell lengths	89.315, 95.619, 47.715
Unit cell angles	90.00, 94.49, 90.00

Refinement procedure

Resolution	90.000 - 2.600
R-factor	0.233 *
Rwork	0.226
R-free	0.26400 *
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	21.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS (0.4)
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.000	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.053	0.300
Number of reflections	23889
<I/σ(I)>	17.9	0.3
Completeness [%]	98.5	97.8
Redundancy	2	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8		pH 8.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	erythrotol	12.5 (%)