1ZAT
Crystal Structure of an Enterococcus faecium peptidoglycan binding protein at 2.4 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979676 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 115.976, 115.976, 68.275 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.920 - 2.400 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.25700 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.920 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 20893 | |
| <I/σ(I)> | 6.4 | 1.5 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 8 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 291 | ammonium sulfate, sodium chloride, sodium acetate, PEG 2000, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
