1ZAR
Crystal Structure of A.fulgidus Rio2 Kinase Complexed With ADP and Manganese Ions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-08 |
Detector | MARRESEARCH |
Wavelength(s) | 0.99997 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 116.328, 44.589, 62.630 |
Unit cell angles | 90.00, 93.88, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.750 |
R-factor | 0.18491 |
Rwork | 0.183 |
R-free | 0.21832 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tqi |
RMSD bond length | 0.012 |
RMSD bond angle | 1.335 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.795 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 32109 | |
Completeness [%] | 98.5 | 87.33 |
Redundancy | 3.9 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.68 | 293 | PEG 900, SODIUM PHOSPHATE, SODIUM CITRATE , pH 3.68, VAPOR DIFFUSION, HANGING DROP, temperature 293K |