1ZAO
Crystal Structure of A.fulgidus Rio2 Kinase Complexed With ATP and Manganese Ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.99997 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.862, 44.365, 62.793 |
| Unit cell angles | 90.00, 94.01, 90.00 |
Refinement procedure
| Resolution | 63.250 - 1.840 |
| R-factor | 0.1835 |
| Rwork | 0.183 |
| R-free | 0.21240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tqi |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.414 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.250 | 1.887 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Number of reflections | 27131 | |
| Completeness [%] | 68.2 | |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.78 | 293 | PEG 900, SODIUM PHOSPHATE, SODIUM CITRATE , pH 3.78, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
