1ZA6

The structure of an antitumor CH2-domain-deleted humanized antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-04
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 2
Unit cell lengths	82.540, 224.200, 166.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.130 - 2.800
R-factor	0.246
Rwork	0.246
R-free	0.29600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1BBJ; PDB ENTRY 1FC1
RMSD bond length	0.013
RMSD bond angle	1.880
Data reduction software	d*TREK (V. 9.0)
Data scaling software	d*TREK (V. 9.0)
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.130	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.152	0.365
Number of reflections	76662
<I/σ(I)>	10.9	5
Completeness [%]	99.9	98
Redundancy	9.06	7.62

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	290	4 M sodium formate, 1.5 mM Triton X-100 detergent, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 290K, pH 7.20