1Z9L

1.7 Angstrom Crystal Structure of the Rat VAP-A MSP Homology Domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-06
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9795, 0.9793, 0.9649
Spacegroup name	P 42 21 2
Unit cell lengths	48.200, 48.200, 112.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.580 - 1.700
R-factor	0.22
Rwork	0.220
R-free	0.25400
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.400
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.790
High resolution limit [Å]	1.700	1.700
Number of reflections	27860
Completeness [%]	98.8	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	283	PEG2000 MME, NaCl, tris buffer, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 283K