1Z9D

Crystal structure of a putative uridylate kinase (UMP-kinase) from Streptococcus pyogenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-26
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	P 21 21 2
Unit cell lengths	161.288, 57.309, 94.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.720 - 2.800
R-factor	0.2151
Rwork	0.215
R-free	0.24700
Structure solution method	SAD aided by MR
RMSD bond length	0.008
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	38306
<I/σ(I)>		2.05
Completeness [%]	93.1	92.9
Redundancy	11.04

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	2M Ammonium sulfate, 0.15M Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K