1Z9C
Crystal structure of OhrR bound to the ohrA promoter: Structure of MarR family protein with operator DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.899, 80.960, 109.340 |
| Unit cell angles | 90.00, 100.20, 90.00 |
Refinement procedure
| Resolution | 59.650 - 2.640 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | EPMR (2.5) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.650 | 2.760 |
| High resolution limit [Å] | 2.640 | 2.640 |
| Rmerge | 0.054 | 0.386 |
| Number of reflections | 47153 | |
| <I/σ(I)> | 11.1 | 2 |
| Completeness [%] | 96.9 | 96.9 |
| Redundancy | 2.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.7 | 298 | pH 3.7, 0.1 M MgCl2 and 5 to 8 % of PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MgCl2 | ||
| 2 | 1 | 1 | PEG 8000 | ||
| 3 | 1 | 2 | MgCl2 | ||
| 4 | 1 | 2 | PEG 8000 |
