1Z96
Crystal structure of the Mud1 UBA domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.771 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 60.601, 60.601, 96.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.890 - 1.800 |
| R-factor | 0.16801 |
| Rwork | 0.167 |
| R-free | 0.19593 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.221 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.868 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.050 | 0.444 |
| Number of reflections | 10214 | |
| <I/σ(I)> | 10.1 | 1.7 |
| Completeness [%] | 99.8 | 99.79 |
| Redundancy | 10.3 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | sodium formate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | sodium formate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
