1Z93

Human Carbonic Anhydrase III:Structural and Kinetic study of Catalysis and Proton Transfer.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2005-01-08
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 65
Unit cell lengths	44.700, 44.700, 222.557
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.100
Rwork	0.205
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1flj
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.170
High resolution limit [Å]	2.100	2.100
Number of reflections	12938
Completeness [%]	88.1	85

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	273	30% PEG 8K, 10mM Tris pH8.0, VAPOR DIFFUSION, HANGING DROP, temperature 273K