1Z8H

CRYSTAL STRUCTURE OF a GDSL-like lipase (ALR1529) FROM NOSTOC SP. PCC 7120 AT 2.02 A RESOLUTION

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-08-31
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9787, 1.0332,0.9784
Spacegroup name	P 21 21 21
Unit cell lengths	64.504, 69.806, 211.028
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.550 - 2.020
R-factor	0.1567
Rwork	0.155
R-free	0.19469
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.469
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.550	2.090
High resolution limit [Å]	2.020	2.020
Number of reflections	62663
<I/σ(I)>	26.76	4.9
Completeness [%]	99.0	95.27
Redundancy	9.6	7.85

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP		277	10% NP_Isopropanol, 0.06M HEPES, 0.04M HEPES_Na, 12% PEG 4000, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K