1Z8D
Crystal Structure of Human Muscle Glycogen Phosphorylase a with AMP and Glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 92.370, 144.000, 59.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.790 - 2.300 |
| Rwork | 0.192 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7gpb |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNX (2000.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.700 | 2.360 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.068 | 0.201 |
| Number of reflections | 36521 | |
| <I/σ(I)> | 11.4 | 5 |
| Completeness [%] | 97.5 | 93.1 |
| Redundancy | 3.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 277 | PEG mme 550, glucose, TRIS, spermine, DTT, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
