1Z7X

X-ray structure of human ribonuclease inhibitor complexed with ribonuclease I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-12
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.99997
Spacegroup name	P 21 21 21
Unit cell lengths	71.338, 107.546, 155.036
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.190 - 1.950
R-factor	0.178
Rwork	0.175
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dfj
RMSD bond length	0.016
RMSD bond angle	1.515
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.190	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.078	0.424
Number of reflections	84446
<I/σ(I)>	16.964	2.939
Completeness [%]	97.0	72.6
Redundancy	6.8	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.2	293	10% MEPEG 2000, 0.020 M SODIUM CITRATE, 0.025 M DTT, 0.001 M AMMONIUM SULFATE, vapor diffusion, HANGING DROP, temperature 293K, pH 4.2