1Z7Q

Crystal structure of the 20s proteasome from yeast in complex with the proteasome activator PA26 from Trypanosome brucei at 3.2 angstroms resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-02-15
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	192.964, 232.127, 296.772
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.800 - 3.220
R-factor	0.263
Rwork	0.263
R-free	0.30800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	one-half of the yeast 20S proteasome (Huber et al. high-resolution structure) pdb entry 1RYP
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.200	3.200
Rmerge	0.112	0.331
Number of reflections	189615
<I/σ(I)>	7	1.9
Completeness [%]	89.1	0.0629
Redundancy	2.7	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.1 M NaHEPES, 40% MPD, 0.2 M NACL, pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 298K