1Z7G
Free human HGPRT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-06-03 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 149.570, 51.800, 117.520 |
| Unit cell angles | 90.00, 105.80, 90.00 |
Refinement procedure
| Resolution | 46.300 - 1.900 |
| R-factor | 0.219 |
| Rwork | 0.213 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hmp |
| RMSD bond length | 1.560 |
| RMSD bond angle | 2.230 |
| Data reduction software | CRYSTAL (CLEAR 1.35) |
| Data scaling software | CrystalClear (V. 1.35 (MSC/RIGAKU)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.300 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.062 | 0.288 |
| Number of reflections | 66957 | |
| <I/σ(I)> | 12.3 | 2.2 |
| Completeness [%] | 95.6 | 79.3 |
| Redundancy | 2.5 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 0.2 M ammonium acetate, 0.1 M sodium acetate, 30% PEG4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
