1Z7E

Crystal structure of full length ArnA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-12-17
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1808
Spacegroup name	P 21 21 21
Unit cell lengths	151.689, 166.227, 261.992
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.000
R-factor	0.2251
Rwork	0.225
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u9j 1yrw
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.079	0.349
Number of reflections	131520
<I/σ(I)>	14.8	3.9
Completeness [%]	99.2	99.8
Redundancy	1.3	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	0.1M MES pH 7.0, 10% Ethylene glycol, 9% PEG 8000, 14 mM 2mercaptoethanol and 10 mM ATP; protein was pre-incubated on ice with 3mM UDP-GlcA and 3 mM MgSO4 , VAPOR DIFFUSION, HANGING DROP, temperature 289K