1Z7A
Crystal structure of probable Polysaccharide deacetylase from Pseudomonas aeruginosa PAO1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-20 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 1 |
| Unit cell lengths | 101.653, 101.773, 100.990 |
| Unit cell angles | 60.71, 61.24, 89.90 |
Refinement procedure
| Resolution | 50.000 - 1.710 |
| R-factor | 0.15224 |
| Rwork | 0.151 |
| R-free | 0.17461 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.213 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.082 | 0.303 |
| Number of reflections | 304779 | |
| <I/σ(I)> | 16.7 | 2.87 |
| Completeness [%] | 71.2 | |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | PEG4K, i-PrOH, NaCitrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
