1Z72
Structure of a putative transcriptional regulator from Streptococcus pneumoniae
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2005-02-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97908, 0.97919 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 116.023, 122.039, 46.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.100 - 1.450 |
| R-factor | 0.15198 |
| Rwork | 0.151 |
| R-free | 0.16709 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.176 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.510 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.400 | |
| Number of reflections | 116194 | |
| <I/σ(I)> | 2.4 | |
| Completeness [%] | 91.5 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | Tacsimate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
