1Z6X
Structure Of Human ADP-Ribosylation Factor 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 64.770, 64.770, 155.559 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.380 - 2.700 |
| R-factor | 0.22419 |
| Rwork | 0.222 |
| R-free | 0.27269 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hur |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.226 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.380 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.098 | 0.357 |
| Number of reflections | 9676 | |
| <I/σ(I)> | 16 | 6 |
| Completeness [%] | 100 | |
| Redundancy | 7.2 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | sodium citrate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
