1Z6R
Crystal structure of Mlc from Escherichia coli
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97637, 0.97866 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 235.950, 74.710, 154.950 |
| Unit cell angles | 90.00, 129.15, 90.00 |
Refinement procedure
| Resolution | 19.940 - 2.700 |
| R-factor | 0.20564 |
| Rwork | 0.203 |
| R-free | 0.26265 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.548 |
| Data scaling software | XDS |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 57176 | |
| <I/σ(I)> | 12.2 | 2 |
| Completeness [%] | 98.4 | 93.6 |
| Redundancy | 7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.6M MgSO4, 100mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
