1Z6O

Crystal Structure of Trichoplusia ni secreted ferritin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	123
Detector technology	CCD
Collection date	2004-04-03
Detector	ADSC QUANTUM 4
Spacegroup name	C 1 2 1
Unit cell lengths	206.880, 145.697, 209.158
Unit cell angles	90.00, 94.93, 90.00

Refinement procedure

Resolution	19.980 - 1.910
R-factor	0.189
Rwork	0.189
R-free	0.19400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mfr
RMSD bond length	0.009
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.850	1.970
High resolution limit [Å]	1.910	1.900
Rmerge	0.065	0.344
Number of reflections	463420
<I/σ(I)>	11.7	2.2
Completeness [%]	97.0	98.7
Redundancy	1.89

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8	277	20 mM Tris, 150 mM NaCl, 0.05% sodium azide, pH 8, spontaneous in storage buffer, temperature 277K, pH 8.00