1Z6I
Crystal structure of the ectodomain of Drosophila transmembrane receptor PGRP-LCa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2005-01-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 105.799, 105.799, 105.799 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.540 - 2.500 |
| R-factor | 0.215 |
| Rwork | 0.213 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.268 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.540 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.062 | 0.558 |
| Number of reflections | 7439 | |
| <I/σ(I)> | 27.44 | 2.42 |
| Completeness [%] | 99.4 | 99.6 |
| Redundancy | 6.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 298 | ammonium sulphate, phosphate citrate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K, temperature 298.0K |
