1Z68

Crystal Structure Of Human Fibroblast Activation Protein alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	98
Detector technology	CCD
Collection date	2002-08-28
Detector	ADSC QUANTUM 4r
Spacegroup name	P 21 21 21
Unit cell lengths	70.295, 152.580, 214.855
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.600
R-factor	0.22619
Rwork	0.223
R-free	0.28257
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1r9m
RMSD bond length	0.010
RMSD bond angle	1.382
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	70612
<I/σ(I)>	12	1.9
Completeness [%]		90.6
Redundancy	3.4	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6		pH 7.6, VAPOR DIFFUSION, SITTING DROP