1Z67
Structure of Homeodomain-like Protein of Unknown Function S4005 from Shigella flexneri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-07 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 57.476, 40.479, 48.333 |
| Unit cell angles | 90.00, 93.78, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.450 |
| R-factor | 0.1643 |
| Rwork | 0.162 |
| R-free | 0.20790 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.427 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.040 | 0.330 |
| Number of reflections | 17111 | |
| <I/σ(I)> | 39.2 | 2.14 |
| Completeness [%] | 86.5 | 26.5 |
| Redundancy | 4.1 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M Bis-Tris, 2 M ammonium sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
