1Z5X
hemipteran ecdysone receptor ligand-binding domain complexed with ponasterone A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 115 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-02-21 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 143.005, 143.005, 84.015 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.480 - 3.070 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Homology model derived from pdb entry 1DKF |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.600 |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 ((MOLREP)) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.480 | 3.110 |
| High resolution limit [Å] | 3.070 | 3.070 |
| Number of reflections | 16756 | |
| <I/σ(I)> | 19.3 | 5.6 |
| Completeness [%] | 99.7 | 99 |
| Redundancy | 11.4 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1 M ammonium phosphate, 4.5 % trehalose, 10 mM DTT, 10 % glycerol, 3 mM ponasterone A, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
