1Z5H

Crystal structures of the Tricorn interacting Factor F3 from Thermoplasma acidophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-16
Detector	MARRESEARCH
Wavelength(s)	1.005
Spacegroup name	P 21 21 2
Unit cell lengths	115.200, 183.300, 105.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.480 - 2.300
R-factor	0.225
Rwork	0.225
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.500	2.440
High resolution limit [Å]	2.300	2.300
Number of reflections	99516
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	PEG 2000, lithium sulphate, TRIS, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K