1Z59

Topoisomerase VI-B, ADP-bound monomer form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-08-04
Detector	ADSC QUANTUM 210
Wavelength(s)	1.100
Spacegroup name	P 21 21 2
Unit cell lengths	94.573, 112.451, 56.079
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.620 - 2.100
R-factor	0.19244
Rwork	0.189
R-free	0.22763
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mu5
RMSD bond length	0.020
RMSD bond angle	1.171
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	35386
<I/σ(I)>	12.1	3.3
Completeness [%]	99.1	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	292	HEPES, NaCl, Mg(HCO2)2, PEG-3350, MgCl2, ADP, pH 7.5, Microbatch, temperature 292K