1Z59
Topoisomerase VI-B, ADP-bound monomer form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.100 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 94.573, 112.451, 56.079 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.620 - 2.100 |
| R-factor | 0.19244 |
| Rwork | 0.189 |
| R-free | 0.22763 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mu5 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.171 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 35386 | |
| <I/σ(I)> | 12.1 | 3.3 |
| Completeness [%] | 99.1 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 292 | HEPES, NaCl, Mg(HCO2)2, PEG-3350, MgCl2, ADP, pH 7.5, Microbatch, temperature 292K |
