1Z58
Crystal structure of a complex of the ribosome large subunit with rapamycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-04-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.93 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 168.500, 404.000, 689.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 3.800 |
R-factor | 0.304 |
Rwork | 0.273 |
R-free | 0.36800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.610 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.940 |
High resolution limit [Å] | 3.800 | 3.800 |
Rmerge | 0.157 | |
Number of reflections | 191012 | |
<I/σ(I)> | 6.06 | |
Completeness [%] | 82.8 | 74.7 |
Redundancy | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7 | 295 | ETHANOL, DIMETHYLHEXANEDIOL, MGCL2,KCL, HEPES, NH4CL, pH 7, EVAPORATION, temperature 295K |
1 | EVAPORATION | 7 | 295 | ETHANOL, DIMETHYLHEXANEDIOL, MGCL2,KCL, HEPES, NH4CL, pH 7, EVAPORATION, temperature 295K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | ETHANOL | ||
10 | 1 | 2 | NH4CL | ||
2 | 1 | 1 | DIMETHYLHEXANEDIOL | ||
3 | 1 | 1 | MGCL2 | ||
4 | 1 | 1 | KCL | ||
5 | 1 | 1 | HEPES | ||
6 | 1 | 1 | NH4CL | ||
7 | 1 | 1 | H2O | ||
8 | 1 | 2 | MGCL2 | ||
9 | 1 | 2 | KCL |