1Z58
Crystal structure of a complex of the ribosome large subunit with rapamycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 168.500, 404.000, 689.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.800 |
| R-factor | 0.304 |
| Rwork | 0.273 |
| R-free | 0.36800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.610 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.940 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.157 | |
| Number of reflections | 191012 | |
| <I/σ(I)> | 6.06 | |
| Completeness [%] | 82.8 | 74.7 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 295 | ETHANOL, DIMETHYLHEXANEDIOL, MGCL2,KCL, HEPES, NH4CL, pH 7, EVAPORATION, temperature 295K |
| 1 | EVAPORATION | 7 | 295 | ETHANOL, DIMETHYLHEXANEDIOL, MGCL2,KCL, HEPES, NH4CL, pH 7, EVAPORATION, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ETHANOL | ||
| 10 | 1 | 2 | NH4CL | ||
| 2 | 1 | 1 | DIMETHYLHEXANEDIOL | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | KCL | ||
| 5 | 1 | 1 | HEPES | ||
| 6 | 1 | 1 | NH4CL | ||
| 7 | 1 | 1 | H2O | ||
| 8 | 1 | 2 | MGCL2 | ||
| 9 | 1 | 2 | KCL |
