1Z4R
Human GCN5 Acetyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-08 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 61 |
| Unit cell lengths | 38.090, 38.090, 186.340 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.980 - 1.740 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ygh |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.980 | 1.740 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.056 | 0.169 |
| Number of reflections | 15315 | |
| <I/σ(I)> | 19.7 | 3.3 |
| Completeness [%] | 87.2 | 87.2 |
| Redundancy | 6.71 | 1.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 300 | 15% (v/v) ethanol, 100 mM Tris, pH 7.0, VAPOR DIFFUSION, temperature 300K |
