1Z4I

Structure of the D41N variant of the human mitochondrial deoxyribonucleotidase in complex with deoxyribouridine 5'-monophosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-07
Detector	ADSC QUANTUM 4
Spacegroup name	P 43 21 2
Unit cell lengths	73.700, 73.700, 106.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.980
R-factor	0.16564
Rwork	0.164
R-free	0.20608
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.690
Data reduction software	MOSFLM
Data scaling software	XDS
Phasing software	CNS
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.100
High resolution limit [Å]	1.980	1.980
Number of reflections	21057
<I/σ(I)>	23.6	7.8
Completeness [%]	100.0	98.8
Redundancy	15.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	PEG8000, potassium dihydrogen phosphate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K