1Z3L
X-Ray Crystal Structure of a Mutant Ribonuclease S (F8Anb)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2003-12-05 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 44.464, 44.464, 97.469 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.970 - 1.800 |
R-factor | 0.197 |
Rwork | 0.197 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rln |
RMSD bond length | 0.004 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.071 | 0.208 |
Number of reflections | 10679 | |
Completeness [%] | 97.8 | 97 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method | 5.75 | 293 | ammonium sulfate, cesium chloride, sodium acetate, pH 5.75, Batch method, temperature 293K |