1Z3G
Crystal structure of complex between Pvs25 and Fab fragment of malaria transmission blocking antibody 2A8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97942 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.262, 61.649, 142.650 |
| Unit cell angles | 90.00, 101.68, 90.00 |
Refinement procedure
| Resolution | 49.150 - 3.300 |
| R-factor | 0.275 |
| Rwork | 0.272 |
| R-free | 0.32700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Pvs25 model 2E8 Fab model |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.775 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.160 | 3.360 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 21703 | |
| <I/σ(I)> | 5.2 | 1.3 |
| Completeness [%] | 95.1 | 87.5 |
| Redundancy | 4.5 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | PEG6000, Lithium Chloride, Citic acid buffer, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
