1Z39
Crystal structure of Trichomonas vaginalis purine nucleoside phosphorylase complexed with 2'-deoxyinosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Wavelength(s) | 0.9766 |
Spacegroup name | P 41 3 2 |
Unit cell lengths | 136.800, 136.800, 136.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.260 - 2.600 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pke |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.260 | 2.760 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 13005 | |
Completeness [%] | 92.8 | 85 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | PEG400, ethylene glycol, magnesium chloride, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |