1Z2W
Crystal structure of mouse Vps29 complexed with Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-06-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.914, 68.953, 60.519 |
| Unit cell angles | 90.00, 106.65, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.17939 |
| Rwork | 0.176 |
| R-free | 0.23742 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z2x |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.134 |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.441 |
| Number of reflections | 30024 | |
| <I/σ(I)> | 12.9 | 3.7 |
| Completeness [%] | 97.6 | 97.6 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.1M Tris, 0.2M NaCl, 20% PEG3000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
