1Z2U
The 1.1A crystallographic structure of ubiquitin-conjugating enzyme (ubc-2) from Caenorhabditis elegans: functional and evolutionary significance
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-17 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.604, 60.457, 43.980 |
| Unit cell angles | 90.00, 106.45, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.100 |
| Rwork | 0.131 |
| R-free | 0.14930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qcq |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.335 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.140 |
| High resolution limit [Å] | 1.100 | 2.370 | 1.100 |
| Rmerge | 0.055 | 0.046 | 0.143 |
| Number of reflections | 58674 | ||
| Completeness [%] | 97.4 | 99.8 | 92.6 |
| Redundancy | 5.5 | 7.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | capillary counter-diffusion | 5 | 277.5 | 7mg/mL protein in 2mM sodium citrate, 10% (v/v) ethanol, 1.5M Sodium Chloride, pH 5.0, capillary counter-diffusion, temperature 277.5K |
