1Z27
Crystal structure of Native Pvs25, an ookinete protein from Plasmodium vivax.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-07-07 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.611, 59.805, 66.747 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.500 - 2.080 |
| R-factor | 0.258 |
| Rwork | 0.258 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Pvs25 model from Methylated Pvs25 structure solved in P21 space group |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.390 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.500 | 2.150 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Number of reflections | 10384 | |
| <I/σ(I)> | 32.5 | 3.95 |
| Completeness [%] | 96.7 | 91.2 |
| Redundancy | 12 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | PEG1500, NaMES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
