1Z1Y
Crystal structure of Methylated Pvs25, an ookinete protein from Plasmodium vivax
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.09524 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.164, 43.700, 65.650 |
| Unit cell angles | 90.00, 103.15, 90.00 |
Refinement procedure
| Resolution | 28.600 - 2.000 |
| R-factor | 0.245 |
| Rwork | 0.244 |
| R-free | 0.27600 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.364 |
| Data reduction software | HKL-2000 |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.600 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.083 | 0.187 |
| Number of reflections | 21346 | |
| <I/σ(I)> | 12.4 | 5.2 |
| Completeness [%] | 98.7 | 98.1 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | PEG1500, sodium accetate, ammonium accetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
